Part:BBa_K4594004:Experience

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Applications of BBa_K4594004

Our literature research revealed that the optimal temperature for the LuxAB-encoded luciferase reaction is 18°C, with rapid inactivation above 25°C [1]. We optimized the experimental conditions, used 0.5 mM IPTG for induction, and observed the results after 10 h. The results were photographed and recorded with a cell phone camera, and the luminescence intensity and OD value were detected using a multifunctional microplate reader.

Luminescence results of optimized luminescent gene circuits.

A: Original camera shot luminescent engineering bacteria.

B: Exposure 15s shot luminescent engineering bacteria.

C: Multifunctional microplate reader luminescence test.

Reference

[1] 杜宗军,王祥红,李海峰,等. 发光细菌的研究和应用[J]. 高技术通讯,2003,13(12):103-106. DOI:10.3321/j.issn:1002-0470.2003.12.022.

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